A blue and green logo

AI-generated content may be incorrect.

ProteanFect Transfection Kit

----- by AURORABOH INC

👉 Inquiries: Contact us at aurorabohsales@gmail.com

Product

PDF version

 

ProteanFect™ Transfection Kit

Cat. NoPT01

ProteanFect™ Transfection Reagent is designed to efficiently transfect even the most challenging cell types, ensuring superior performance across a broad range of cells. It also excels at delivering high molecular weight nucleic acids or co-transfecting multiple nucleic acids with exceptional efficiency. For research use only.

Number

Standard

PT01-0010B

0.1 mL

PT01-0030B

0.3 mL

PT01-0050B

0.5 mL

PT01-0100B

1.0 mL

PT01-0500B

5.0 mL

 

Documents

Datasheet                  Protocol

 

Components & Storage

The kit is shipped on dry ice. Once received, store the components as indicated below. The kit includes positive control samples with EGFP-encoding mRNA and pDNA (plasmid DNA) to verify transfection efficiency.

Components

Storage

Reagent A

2-8°C

Reagent B

-20°C(Avoid repeated freeze-thaw cycles)

EGFP mRNA (1 µg/µL)

-80°C(Avoid repeated freeze-thaw cycles)

GFP pDNA (0.5 µg/µL)

-20°C(Avoid repeated freeze-thaw cycles)

 

Compatible Cell Lines

 

Cell Lines Successfully Transfected Using ProteanFect™ Transfection Kit

Cell lines

Tested Nucleic Acid Types for Transfection

Jurkat T (human T lymphoblastic leukemia cells)

pDNA, mRNA, siRNA

LX-2 (human hepatic stellate cells)

pDNA, mRNA, siRNA

HepG2 (human liver tumor cells)

pDNA, mRNA, siRNA

THP-1 (human acute monocytic leukemia cell line)

mRNA, siRNA

Raji (human Burkitt lymphoma cells)

mRNA, siRNA

K562 (human chronic myeloid leukemia cells)

pDNA, mRNA, siRNA

MOLT-16 (human T lymphoblastic leukemia cells)

mRNA, siRNA

SH-SY5Y (human neuroblastoma cells)

pDNA, mRNA, siRNA

U2OS (human osteosarcoma cells)

pDNA, mRNA, siRNA

U937 (human lymphoma cell line)

mRNA, siRNA

HFF (human foreskin fibroblasts)

pDNA, mRNA, siRNA

MC38 (mouse colon cancer cells)

mRNA, siRNA

RAW264.7 (mouse mononuclear macrophage leukemia cells)

mRNA, siRNA

LLC (mouse Lewis lung cancer cells)

pDNA, mRNA, siRNA

C2C12 (mouse myoblasts)

pDNA, mRNA, siRNA

COS7 (African green monkey kidney fibroblast-like cells)

pDNA, mRNA, siRNA