ProteanFect Transfection Kit

----- by AURORABOH INC

👉 Inquiries: Contact us at aurorabohsales@gmail.com

Product

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ProteanFect™ Max Transfection Kit

Cat. NoPT02

ProteanFect™ Max Transfection Kit is designed to efficiently transfect a wide range of primary cells with high efficiency, while maintaining an excellent safety profile. It facilitates easy scale-up and meets the demands of high-throughput experiments. Additionally, it is user-friendly, making transfection of primary cells as straightforward as transfecting HEK293T cells. For research use only.

Number	Standard
PT02-0010B	0.1 mL
PT02-0020B	0.2 mL
PT02-0050B	0.5 mL
PT02-0100B	1 mL
PT02-0500B	5 mL

Documents

Datasheet Protocol

Components & Storage

The kit is shipped on dry ice. Once received, store the components as indicated below. The kit includes positive control samples with EGFP-encoding mRNA and plasmid DNA (pDNA) to verify transfection efficiency.

Components	Storage
Reagent A	2-8°C
Reagent B	-20°C(Avoid repeated freeze-thaw cycles)
Reagent C	2-8°C
EGFP mRNA (1 µg/µL)	-80°C (Avoid repeated freeze-thaw cycles)
GFP pDNA (0.5 µg/µL)	-20°C (Avoid repeated freeze-thaw cycles)

Compatible Cell Lines

Primary Cells and Cell Lines Successfully Transfected Using ProteanFect™ Max Transfection Kit

Cell lines	Tested Nucleic Acid Types for Transfection
Human primary T cells	mRNA, siRNA
Human primary NK cells	mRNA, siRNA
Human primary monocytes	mRNA, siRNA
Human primary CD34+ hematopoietic stem cells	mRNA, siRNA
Mouse primary cardiomyocytes	mRNA, siRNA
Mouse primary neurons	pDNA, mRNA, siRNA
Mouse primary glial cells	pDNA, mRNA, siRNA
Mouse primary T cells	mRNA, siRNA
Mouse primary NK cells	mRNA, siRNA
Large yellow croaker primary mesenchymal stem cells	mRNA, siRNA
Jurkat T (human T lymphoblastic leukemia cells)	pDNA, mRNA, siRNA
LX-2 (human hepatic stellate cells)	pDNA , mRNA, siRNA
HepG2 (human liver tumor cells)	pDNA, mRNA, siRNA
THP-1 (human acute monocytic leukemia cell line)	mRNA, siRNA
Raji (human Burkitt lymphoma cells)	mRNA, siRNA
K562 (human chronic myeloid leukemia cells)	pDNA, mRNA, siRNA
MOLT-16 (human T lymphoblastic leukemia cells)	mRNA, siRNA
SH-SY5Y (human neuroblastoma cells)	pDNA, mRNA, siRNA
U2OS (human osteosarcoma cells)	pDNA, mRNA, siRNA
HFF (human foreskin fibroblasts)	mRNA, siRNA
HEK-293 (human embryonic kidney cell line)	pDNA, mRNA, siRNA
MC38 (mouse colon cancer cells)	mRNA, siRNA
RAW264.7 (mouse mononuclear macrophage leukemia cells)	mRNA, siRNA
LLC (mouse Lewis lung cancer cells)	pDNA, mRNA, siRNA
C2C12 (mouse myoblasts)	pDNA, mRNA, siRNA
COS7 (African green monkey kidney fibroblast-like cells)	pDNA, mRNA, siRNA