ProteanFect Transfection Kit

----- by AURORABOH INC

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Product

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ProteanFect™ Max Mouse Immunocyte Transfection Kit

Cat. NoPT03

ProteanFect™ Max Mouse Immunocyte Transfection Kit is designed to efficiently transfect primary mouse immune cells, including T cells and NK cells. It achieves high transfection efficiency while maintaining minimal toxicity for these fragile immune cells. For research use only.

Number	Standard
PT03-0010B	0.1 mL
PT03-0020B	0.2 mL
PT03-0050B	0.5 mL
PT03-0100B	1 mL
PT03-0500B	5 mL

Documents

Datasheet Protocol

Components & Storage

The kit is shipped on dry ice. Once received, store the components as indicated below. The kit includes positive control samples with EGFP-encoding mRNA to verify transfection efficiency.

Components	Storage
Reagent A (for Mouse Immunocyte)	2-8°C
Reagent B (for Mouse Immunocyte)	-20°C(Avoid repeated freeze-thaw cycles
Reagent C (for Mouse Immunocyte)	2-8°C
EGFP mRNA (1 µg/µL)	-80°C (Avoid repeated freeze-thaw cycles)

Compatible Cell Lines

Primary Cells Successfully Transfected Using ProteanFect™ Max Mouse Immunocyte Transfection Kit

Cell lines	Tested Nucleic Acid Types for Transfection
Mouse primary T cells	mRNA, siRNA
Mouse primary NK cells	mRNA, siRNA
Mouse primary γδ T cells	mRNA, siRNA
Mouse primary Treg cells	mRNA, siRNA
Mouse primary CD4+ cells	mRNA, siRNA
Mouse primary CD8+ cells	mRNA, siRNA