ProteanFect Transfection Kit

----- by AURORABOH INC

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Product

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ProteanFect™ CRISPRMax Gene Editing Transfection Kit

Cat. NoPT05

ProteanFect™ CRISPRMax Gene Editing Transfection Kit offers a non-viral, non-electroporation, and non-liposomal transfection system utilizing engineered mammalian proteins. This innovative design achieves high transfection efficiency while maintaining a superior safety profile. The ProteanFect™ CRISPRMax-Cas9 mRNA Gene Editing Kit is specifically designed for effective gene editing by delivering Cas9 mRNA and sgRNA into primary cells and difficult-to-transfect cell lines. Additionally, it excels in co-transfecting sgRNAs targeting different genes, enabling simultaneous knockout of multiple genes.

Number	Standard
PT05-0010B	0.1 mL
PT05-0020B	0.2 mL
PT05-0050B	0.5 mL
PT05-0100B	1 mL
PT05-0500B	5 mL

Documents

Datasheet Protocol

Components & Storage

The kit is shipped on dry ice. Once received, store the components as indicated below. The kit includes positive control samples with EGFP-encoding mRNA to verify transfection efficiency as well as single-guide RNA (sgRNA) targeting the human TRAC gene.

Components	Storage
Reagent A (for CRISPR-Cas9 mRNA)	2-8℃
Reagent B (for CRISPR-Cas9 mRNA)	-20°C(Avoid repeated freeze-thaw cycles
Reagent C (for CRISPR-Cas9 mRNA)	2-8°C
EGFP mRNA (1 µg/µL)	-80°C (Avoid repeated freeze-thaw cycles)
Human TRAC-sgRNA (1 µg / µL)	-80°C (Avoid repeated freeze-thaw cycles)

Compatible Cell Lines

Primary Cells and Cell Lines Compatible with ProteanFect™ CRISPRMax Gene Editing Transfection Kit

Cell lines	Tested Nucleic Acid Types for Transfection
Human primary T cells	sgRNA
Human primary NK cells	sgRNA
Human primary monocytes	sgRNA
Human primary CD34+ hematopoietic stem cells	sgRNA
Mouse primary cardiomyocytes	sgRNA
Mouse primary neurons	sgRNA
Mouse primary glial cells	sgRNA
Mouse primary T cells	sgRNA
Mouse primary NK cells	sgRNA
Large yellow croaker primary mesenchymal stem cells	sgRNA
Jurkat T (human T lymphoblastic leukemia cells)	sgRNA
LX-2 (human hepatic stellate cells)	sgRNA
HepG2 (human liver tumor cells)	sgRNA
THP-1 (human acute monocytic leukemia cell line)	sgRNA
Raji (human Burkitt lymphoma cells)	sgRNA
K562 (human chronic myeloid leukemia cells)	sgRNA
MOLT-16 (human T lymphoblastic leukemia cells)	sgRNA
SH-SY5Y (human neuroblastoma cells)	sgRNA
U2OS (human osteosarcoma cells)	sgRNA
U937 (human lymphoma cell line)	sgRNA
HFF (human foreskin fibroblasts)	sgRNA
HEK-293 (human embryonic kidney cell line)	sgRNA
MC38 (mouse colon cancer cells)	sgRNA
RAW264.7 (mouse mononuclear macrophage leukemia cells)	sgRNA
LLC (mouse Lewis lung cancer cells)	sgRNA
C2C12 (mouse myoblasts)	sgRNA
COS7 (African green monkey kidney fibroblast-like cells)	sgRNA