ProteanFect Transfection Kit

----- by AURORABOH INC

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Product

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ProteanFect™ CRISPRMax Mouse Immunocyte Gene Editing Transfection Kit

Cat. NoPT06

ProteanFect™ CRISPRMax Mouse Immunocyte Gene Editing Transfection Kit offers a non-viral, non-electroporation, and non-liposomal transfection system utilizing engineered mammalian proteins. This innovative design achieves high transfection efficiency while maintaining a superior safety profile. The ProteanFectTM CRISPRMax-Cas9 mRNA Mouse Immunocyte Gene Editing Kit is specifically designed for effective gene editing by delivering Cas9 mRNA and sgRNA into primary mouse immune cells. It excels in engineering sensitive mouse immune cells, including T cells, NK cells, and γδ T cells.

Number	Standard
PT06-0010B	0.1 mL
PT06-0020B	0.2 mL
PT06-0050B	0.5 mL
PT06-0100B	1 mL
PT06-0500B	5 mL

Documents

Datasheet Protocol

Components & Storage

The kit is shipped on dry ice. Once received, store the components as indicated below. The kit includes positive control samples with EGFP-encoding mRNA to verify transfection efficiency as well as single-guide RNA (sgRNA) targeting the mouse Trac gene.

Components	Storage
Reagent A (for Mouse Immunocyte CRISPR-Cas9 mRNA)	2-8℃
Reagent B (for Mouse Immunocyte CRISPR-Cas9 mRNA)	-20°C(Avoid repeated freeze-thaw cycles
Reagent C (for Mouse Immunocyte CRISPR-Cas9 mRNA)	2-8°C
EGFP mRNA (1 µg/µL)	-80°C (Avoid repeated freeze-thaw cycles)
Mouse Trac-sgRNA (1 µg / µL)	-80°C (Avoid repeated freeze-thaw cycles)

Compatible Cell Lines

Cell Lines Compatible with ProteanFect™ CRISPRMax Mouse Immunocyte Gene Editing Transfection Kit

Cell lines	Tested Nucleic Acid Types for Transfection
Mouse primary T cells	sgRNA
Mouse primary NK cells	sgRNA
Mouse primary γδ T cells	sgRNA
Mouse primary Treg cells	sgRNA
Mouse primary CD4+ cells	sgRNA
Mouse primary CD8+ cells	sgRNA